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Norway spruce chloroplast ultrastructure - heterogeneity within a needle.
Glanc, Natália ; Lhotáková, Zuzana (advisor) ; Kutík, Jaromír (referee)
6 Abstract Temperate forests serve as long term carbon storage and are affected by increasing carbon dioxide (CO2) concentration in the atmosphere. Norway spruce (Picea abies (L.) Karst.) is the most abundant conifer in the forests of the Czech Republic, therefore I studied the response of its photosynthetic aparatus to elevated CO2 concentration. The aim of my thesis was to analyze the impact of CO2 concentration on chloroplast ultrastructure in both shaded and exposed needles, focusing on the volume density of starch in the median cross-sections of mesophyll cell chloroplasts. The next aim of the study was to test whether the chloroplasts of the first subepidermal layer of mesophyll are representative for the whole needle with respect to starch volume density. The study was performed on eleven years-old Norway spruce trees that had been exposed to ambient or elevated concentration of CO2 for six years; the experiment had been carried out at the Bílý Kříž experimental station in the Beskids Mountains in cultivation chambers with automatically adjustable windows. First year needles of trees grown under abient (382-395ppm) or elevated (700 ppm) CO2 concentration were collected in October 2011. The needles were used to prepare ultrathin sections and the images of median chloroplast cross-sections were...
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Study of quantitative parameters of Norway spruce needle structure under the effect of elevated CO₂ concentration and different irradiance
Kubínová, Zuzana ; Lhotáková, Zuzana (advisor) ; Urban, Otmar (referee) ; Gebauer, Roman (referee)
Zuzana Kubínová: Study of Quantitative Parameters of Norway Spruce Needle Structure under the Effect of Elevated CO2 Concentration and Different Irradiance, Doctoral Thesis, Prague 2019 Abstract Atmospheric concentration of CO2 is increasing, while its influence on plants is still not fully elucidated. Norway spruce (Picea abies L. Karst.) is an abundant conifer tree in European temperate and boreal forests, which behave as carbon sink in the global carbon cycle. The physiological response to elevated CO2 concentration may be interconnected with changes in leaf anatomy and morphology. Needle structure is also determined by other factors in addition to CO2 concentration, irradiance being the most important one. Thus, effect of irradiance was also included in our studies. The effects of elevated CO2 concentration and irradiance on Norway spruce needle structure were studied using new applications of well-established quantitative methods and novel methods enabling effective and unbiased analysis of needle structural traits. The General Procrustes analysis showed to be effective for needle shape on cross section comparison and the disector method proved to be suitable for chloroplast number estimates. The influence of elevated CO2 concentration and different irradiance on needle structure was studied at two...
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Ultrastrucutre of beech chloroplasts under the elevated CO2 concentration and different irradiation
Vrbová, Anna ; Albrechtová, Jana (advisor) ; Kutík, Jaromír (referee)
Forest stands may act as important carbon storage places - sinks, due to carbon allocation into both the plant biomass in the process of photosynthesis and the soil. Enhancement of CO2 concentration affects a whole range of plant physiological processes and, thus, it is necessary to study its effect on photosynthetic apparatus - leaf anatomical structure and chloroplast ultrastructure. The first aim of the Thesis was to evaluate changes in chloroplast ultrastructure of common beech (Fagus sylvatica L.) under the effects of both elevated CO2 concentration and different irradiance. The second aim was to evaluate if the anatomical parameters obtained from the middle part of the leaf are representative for the whole leaf blade. The trees were grown in glass domes at the Bílý Kříž experimental site in the Beskids Mountains (Czech Republic), owned by the CzechGlobe Institute. Leaves were sampled in 2010 from juvenile trees, which were planted in 2005 being 5-year old and cultivated since then in ambient (AC; 390 micromol/mol) and elevated (EC; 700 micromol/mol) CO2 concentrations. The EC effect was recorded to be an increased proportion of starch grains in the chloroplast median section and decreased proportion of of intergranal thylakoids (IGT) while the ratio of granal to intergranal thylakoids...
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Study of quantitative parameters of Norway spruce needle structure under the effect of elevated CO₂ concentration and different irradiance
Kubínová, Zuzana ; Lhotáková, Zuzana (advisor) ; Urban, Otmar (referee) ; Gebauer, Roman (referee)
Zuzana Kubínová: Study of Quantitative Parameters of Norway Spruce Needle Structure under the Effect of Elevated CO2 Concentration and Different Irradiance, Doctoral Thesis, Prague 2019 Abstract Atmospheric concentration of CO2 is increasing, while its influence on plants is still not fully elucidated. Norway spruce (Picea abies L. Karst.) is an abundant conifer tree in European temperate and boreal forests, which behave as carbon sink in the global carbon cycle. The physiological response to elevated CO2 concentration may be interconnected with changes in leaf anatomy and morphology. Needle structure is also determined by other factors in addition to CO2 concentration, irradiance being the most important one. Thus, effect of irradiance was also included in our studies. The effects of elevated CO2 concentration and irradiance on Norway spruce needle structure were studied using new applications of well-established quantitative methods and novel methods enabling effective and unbiased analysis of needle structural traits. The General Procrustes analysis showed to be effective for needle shape on cross section comparison and the disector method proved to be suitable for chloroplast number estimates. The influence of elevated CO2 concentration and different irradiance on needle structure was studied at two...
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|
|
Norway spruce chloroplast ultrastructure - heterogeneity within a needle.
Glanc, Natália ; Lhotáková, Zuzana (advisor) ; Kutík, Jaromír (referee)
6 Abstract Temperate forests serve as long term carbon storage and are affected by increasing carbon dioxide (CO2) concentration in the atmosphere. Norway spruce (Picea abies (L.) Karst.) is the most abundant conifer in the forests of the Czech Republic, therefore I studied the response of its photosynthetic aparatus to elevated CO2 concentration. The aim of my thesis was to analyze the impact of CO2 concentration on chloroplast ultrastructure in both shaded and exposed needles, focusing on the volume density of starch in the median cross-sections of mesophyll cell chloroplasts. The next aim of the study was to test whether the chloroplasts of the first subepidermal layer of mesophyll are representative for the whole needle with respect to starch volume density. The study was performed on eleven years-old Norway spruce trees that had been exposed to ambient or elevated concentration of CO2 for six years; the experiment had been carried out at the Bílý Kříž experimental station in the Beskids Mountains in cultivation chambers with automatically adjustable windows. First year needles of trees grown under abient (382-395ppm) or elevated (700 ppm) CO2 concentration were collected in October 2011. The needles were used to prepare ultrathin sections and the images of median chloroplast cross-sections were...
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Ultrastrucutre of beech chloroplasts under the elevated CO2 concentration and different irradiation
Vrbová, Anna ; Albrechtová, Jana (advisor) ; Kutík, Jaromír (referee)
Forest stands may act as important carbon storage places - sinks, due to carbon allocation into both the plant biomass in the process of photosynthesis and the soil. Enhancement of CO2 concentration affects a whole range of plant physiological processes and, thus, it is necessary to study its effect on photosynthetic apparatus - leaf anatomical structure and chloroplast ultrastructure. The first aim of the Thesis was to evaluate changes in chloroplast ultrastructure of common beech (Fagus sylvatica L.) under the effects of both elevated CO2 concentration and different irradiance. The second aim was to evaluate if the anatomical parameters obtained from the middle part of the leaf are representative for the whole leaf blade. The trees were grown in glass domes at the Bílý Kříž experimental site in the Beskids Mountains (Czech Republic), owned by the CzechGlobe Institute. Leaves were sampled in 2010 from juvenile trees, which were planted in 2005 being 5-year old and cultivated since then in ambient (AC; 390 micromol/mol) and elevated (EC; 700 micromol/mol) CO2 concentrations. The EC effect was recorded to be an increased proportion of starch grains in the chloroplast median section and decreased proportion of of intergranal thylakoids (IGT) while the ratio of granal to intergranal thylakoids...
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